A heatmap is a color-coded grid for a numeric matrix. Rows and columns stay as separate categories, and the color scale shows high, low, similar and unusual values without reading every number. Researchers use heatmaps for gene expression, correlation, model errors, activity and business data.
Sequential palettes suit values that rise from low to high. Diverging palettes suit values around a meaningful midpoint, such as zero, change from baseline or a Z-score. The legend is part of the evidence, so it must say what the colors mean.
Clustering can reorder rows or columns so similar profiles sit together. A dendrogram records that order, but it does not prove biological or causal groups. Read clusters alongside the study design and the source data.
Matrix
Rows and columns define the observations or variables being compared.
Color scale
A legend maps numeric values to color and identifies the midpoint.
Normalization
Row or column Z-scores change the question from magnitude to relative pattern.
Clustering
A distance metric and a linkage rule decide which similar profiles sit together.









